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antip35 25  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc antip35 25
    Antip35 25, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 166 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antip35+25/p35%2F25+Rabbit+mAb/pm41894510-283-104-106
    Average 95 stars, based on 166 article reviews
    antip35 25 - by Bioz Stars, 2026-09
    95/100 stars

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    other:

    Article Title: TMEM59 deficiency activates chaperone-mediated autophagy and ameliorates disease-like pathologies in tauopathy model mice.
    Article Snippet: The blots were immunoblotted with primary antibodies, including: anti-β-actin (CST, 8457S, 1:2000), anti-ATF6 (Abcam, ab227830, 1:1000), anti-BiP (CST, C50B12, 1:1000), antiCDK5 (Santa Cruz, sc-173, 1:1000), anti-Flag (Proteintech, 20543- 1-AP, 1:1000), anti-GAPDH (Abways, AB0038, 1:5000), anti-GFAP (Cell Signaling Technology, 3670S, 1:1000), anti-GFP (Proteintech, 50430-2-AP, 1:1000), anti-GluA2 (CST, 13607S, 1:1000), anti-GSK3β (Proteintech, 51065-1-AP, 1:1000), anti-HA (Proteintech, S1064-2AP, 1:1000), anti-HSC70 (Proteintech, 10654-1-AP, 1:1000), anti-Iba1 (Wako, 016-20001, 1:500), anti-LAMP2A (Abcam, ab18528, 1:1000), anti-LC3B (CST, 3868, 1:500), anti-myc (CST, 2276S, 1:1000), antip35/25 (CST, 2680, 1:1000), anti-PERK (CST, D11A8, 1:1000), antiPP1α (Thermo, 43-8100, 1:1000), anti-PP2A-α (ABclonal, A6702, 1:1000), anti-PP2B-Aα (Santa Cruz, sc-17808, 1:1000), anti-pPERK (CST, 16F8, 1:1000), anti-pS202/T205 tau (AT8, Invitrogen, MN1020, 1:500), anti-pS212 tau (Biosource, 44-740G, 1:1000), anti-pS396 tau (Invitrogen, 44752G, 1:1000), anti-pT216-GSK3β (Proteintech, 51065-1-AP, 1:1000), anti-total tau (Tau5, Invitrogen, AHB0042, 1:1000), anti-TMEM59 (ABclonal, WG-03224D, 1:1000), and antiXBP1 (Abcam, ab220783, 1:1000).

    Western Blot:

    Article Title: A small-molecule stabilizer of the calpastatin-calpain-2 complex restores mitochondrial function and mitigates neurodegeneration.
    Article Snippet: CHIR99021 (S2924) was from Selleckchem (Houston, TX, USA). .. The antibodies used for the Western blot are listed as follows: Anti–DARPP- 32 (ab40801, Abcam, Cambridge, UK, 1:3000), antiBDNF (ab108319, Abcam, 1:1000), anti- ATPB (ATP synthase, beta subunit) (17247- 1- AP, Proteintech, 1:3000), anti- EEA1 (28347- 1- AP, Proteintech, 1:3000), anti- tau antibody (10274- 1- AP, Proteintech, 1:3000), anti–phospho- tau (Ser202 and Thr205) (Thermo Fisher Scientific, clone AT8, 1:1000), anti- GSK3 (05- 412, MilliporeSigma, 1:1000), anti- Htt protein (MAB5374, clone EM48, MilliporeSigma, 1:1000), anti–β- actin (A1978, MilliporeSigma, 1:10000), anti- DLP1 (611113, BD Bioscience, Franklin Lakes, NJ, USA, 1:2000), antiPSD95 (2507, Cell Signaling Technology, Danvers, MA, USA, 1:5000), anti–Drp1- pS616 (3455S, Cell Signaling Technology, 1:1000), antip35/25 (2680, Cell Signaling Technology, 1:1000), anti- CAST (4146, Cell Signaling Technology, 1:2000), anti–calpain- 2 (2539, Cell Signaling Technology, 1:1000), anti–calpain- 1 (2556, Cell Signaling Technology, 1:1000), anti–α- spectrin- II (sc- 48382, Santa Cruz Biotechnology, Dallas, TX, USA, 1:500), and anti- enolase (sc- 15343, Santa Cruz Biotechnology, 1:2000), and horseradish peroxidase (HRP)–conjugated anti- rabbit or anti- mouse IgG (31430/31460, Thermo Fisher Scientific, 1:5000). ..



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    Fig. 2 DNase II deficiency promotes tau phosphorylation in primary hippocampal neurons through activation of the CDK5, CaMKII and PP2A signaling pathway. a Representative images of AT8 (red) and pThr231 (red) fluorescence staining in primary hippocampal neurons infected with shDNase2a or shCON. Scale bars, 10 μm. b Quantification of AT8 and pThr231 fluorescent areas in (a) by Image J software. c Western blotting of AT8 and pThr231 in the primary hippocampal neurons infected with shDNase2a or shCON. d Quantitation of the levels of AT8 and pThr231 in (c). e Western blotting of CDK5, Calpain2, <t>p25,</t> p35, CaMKII-α, p-CaMKII-α, PP2A, p-PP2A, CIP2A, GSK-3β, p-GSK-3β, Chk1, p-Chk1, Chk2 and p-Chk2 in the primary hippocampal neurons infected with shDNase2a or shCON. f Quantitation of the bands in (e) by the Image J software. g Representative images of pSer416 (cyan) fluorescence staining in primary hippocampal neurons infected with shDNase2a or shCON. Scale bars, 10 μm. h Quantification of pSer416 fluorescent area in (g) by the Image J software. i Western blotting of pSer416 in the primary hippocampal neurons infected with shDNase2a or shCON. j Quantitation of the levels of pSer416 in (i). In a, c, e, g and i, data are representative of three independent experiments. In b, d, f, h, and j, data were pooled from three independent experiments. Mean ± SEM, unpaired t-test with two-tailed P values was used for statistical analysis. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, not significant
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    Fig. 2 DNase II deficiency promotes tau phosphorylation in primary hippocampal neurons through activation of the CDK5, CaMKII and PP2A signaling pathway. a Representative images of AT8 (red) and pThr231 (red) fluorescence staining in primary hippocampal neurons infected with shDNase2a or shCON. Scale bars, 10 μm. b Quantification of AT8 and pThr231 fluorescent areas in (a) by Image J software. c Western blotting of AT8 and pThr231 in the primary hippocampal neurons infected with shDNase2a or shCON. d Quantitation of the levels of AT8 and pThr231 in (c). e Western blotting of CDK5, Calpain2, <t>p25,</t> p35, CaMKII-α, p-CaMKII-α, PP2A, p-PP2A, CIP2A, GSK-3β, p-GSK-3β, Chk1, p-Chk1, Chk2 and p-Chk2 in the primary hippocampal neurons infected with shDNase2a or shCON. f Quantitation of the bands in (e) by the Image J software. g Representative images of pSer416 (cyan) fluorescence staining in primary hippocampal neurons infected with shDNase2a or shCON. Scale bars, 10 μm. h Quantification of pSer416 fluorescent area in (g) by the Image J software. i Western blotting of pSer416 in the primary hippocampal neurons infected with shDNase2a or shCON. j Quantitation of the levels of pSer416 in (i). In a, c, e, g and i, data are representative of three independent experiments. In b, d, f, h, and j, data were pooled from three independent experiments. Mean ± SEM, unpaired t-test with two-tailed P values was used for statistical analysis. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, not significant
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    Fig. 2 DNase II deficiency promotes tau phosphorylation in primary hippocampal neurons through activation of the CDK5, CaMKII and PP2A signaling pathway. a Representative images of AT8 (red) and pThr231 (red) fluorescence staining in primary hippocampal neurons infected with shDNase2a or shCON. Scale bars, 10 μm. b Quantification of AT8 and pThr231 fluorescent areas in (a) by Image J software. c Western blotting of AT8 and pThr231 in the primary hippocampal neurons infected with shDNase2a or shCON. d Quantitation of the levels of AT8 and pThr231 in (c). e Western blotting of CDK5, Calpain2, <t>p25,</t> p35, CaMKII-α, p-CaMKII-α, PP2A, p-PP2A, CIP2A, GSK-3β, p-GSK-3β, Chk1, p-Chk1, Chk2 and p-Chk2 in the primary hippocampal neurons infected with shDNase2a or shCON. f Quantitation of the bands in (e) by the Image J software. g Representative images of pSer416 (cyan) fluorescence staining in primary hippocampal neurons infected with shDNase2a or shCON. Scale bars, 10 μm. h Quantification of pSer416 fluorescent area in (g) by the Image J software. i Western blotting of pSer416 in the primary hippocampal neurons infected with shDNase2a or shCON. j Quantitation of the levels of pSer416 in (i). In a, c, e, g and i, data are representative of three independent experiments. In b, d, f, h, and j, data were pooled from three independent experiments. Mean ± SEM, unpaired t-test with two-tailed P values was used for statistical analysis. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, not significant
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    Image Search Results


    Fig. 2 DNase II deficiency promotes tau phosphorylation in primary hippocampal neurons through activation of the CDK5, CaMKII and PP2A signaling pathway. a Representative images of AT8 (red) and pThr231 (red) fluorescence staining in primary hippocampal neurons infected with shDNase2a or shCON. Scale bars, 10 μm. b Quantification of AT8 and pThr231 fluorescent areas in (a) by Image J software. c Western blotting of AT8 and pThr231 in the primary hippocampal neurons infected with shDNase2a or shCON. d Quantitation of the levels of AT8 and pThr231 in (c). e Western blotting of CDK5, Calpain2, p25, p35, CaMKII-α, p-CaMKII-α, PP2A, p-PP2A, CIP2A, GSK-3β, p-GSK-3β, Chk1, p-Chk1, Chk2 and p-Chk2 in the primary hippocampal neurons infected with shDNase2a or shCON. f Quantitation of the bands in (e) by the Image J software. g Representative images of pSer416 (cyan) fluorescence staining in primary hippocampal neurons infected with shDNase2a or shCON. Scale bars, 10 μm. h Quantification of pSer416 fluorescent area in (g) by the Image J software. i Western blotting of pSer416 in the primary hippocampal neurons infected with shDNase2a or shCON. j Quantitation of the levels of pSer416 in (i). In a, c, e, g and i, data are representative of three independent experiments. In b, d, f, h, and j, data were pooled from three independent experiments. Mean ± SEM, unpaired t-test with two-tailed P values was used for statistical analysis. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, not significant

    Journal: Translational neurodegeneration

    Article Title: Neuronal double-stranded DNA accumulation induced by DNase II deficiency drives tau phosphorylation and neurodegeneration.

    doi: 10.1186/s40035-024-00427-8

    Figure Lengend Snippet: Fig. 2 DNase II deficiency promotes tau phosphorylation in primary hippocampal neurons through activation of the CDK5, CaMKII and PP2A signaling pathway. a Representative images of AT8 (red) and pThr231 (red) fluorescence staining in primary hippocampal neurons infected with shDNase2a or shCON. Scale bars, 10 μm. b Quantification of AT8 and pThr231 fluorescent areas in (a) by Image J software. c Western blotting of AT8 and pThr231 in the primary hippocampal neurons infected with shDNase2a or shCON. d Quantitation of the levels of AT8 and pThr231 in (c). e Western blotting of CDK5, Calpain2, p25, p35, CaMKII-α, p-CaMKII-α, PP2A, p-PP2A, CIP2A, GSK-3β, p-GSK-3β, Chk1, p-Chk1, Chk2 and p-Chk2 in the primary hippocampal neurons infected with shDNase2a or shCON. f Quantitation of the bands in (e) by the Image J software. g Representative images of pSer416 (cyan) fluorescence staining in primary hippocampal neurons infected with shDNase2a or shCON. Scale bars, 10 μm. h Quantification of pSer416 fluorescent area in (g) by the Image J software. i Western blotting of pSer416 in the primary hippocampal neurons infected with shDNase2a or shCON. j Quantitation of the levels of pSer416 in (i). In a, c, e, g and i, data are representative of three independent experiments. In b, d, f, h, and j, data were pooled from three independent experiments. Mean ± SEM, unpaired t-test with two-tailed P values was used for statistical analysis. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, not significant

    Article Snippet: The following primary antibodies were used for Western blotting: anti-β-actin (Abcam, #ab8226, 1:1000), anti-DNase II (Proteintech, #15934-1-AP, 1:500), antiphospho-tau (Ser202, Thr205) (Invitrogen, #MN1020, 1:500), anti-phospho-tau (Thr231) (Beyotime, #AF1951, 1:500), anti-phospho-tau (Ser416) (Abcam, #ab119391, 1:1000), anti-CDK5 (Abcam, #ab40773, 1:2000), antip35/p25 (Cell Signaling Technology, #2680, 1:1000), anti-PSD95 (Abcam, #ab238135, 1:1000), anti-synaptophysin (Abcam, #ab32127, 1:1000), anti-interferon regulatory factor 3 (IRF3) (Beyotime, #AF2485, 1:1000), anti-phospho-IRF3 (Ser396) (Cell Signaling Technology, #29047, 1:1000), anti-TANK-binding kinase 1 (TBK1)

    Techniques: Phospho-proteomics, Activation Assay, Fluorescence, Staining, Infection, Software, Western Blot, Quantitation Assay, Two Tailed Test